PCR studies on the potential sites for latency of BHV-4 in calves

Vet Res Commun. 1998 Apr;22(3):209-16. doi: 10.1023/a:1006029523226.


The aim of the study was to examine various tissues of experimentally infected calves for the BHV-4 genome so as to detect in which cells the virus persists during the latent phase of the infection. The presence of the bovine herpesvirus type 4 genome was detected by a nested PCR in a variety of tissues collected from two susceptible calves experimentally infected 62 days earlier. Mild clinical signs of bronchitis, an elevated body temperature for 2-3 days, and a slightly increased number of blood leukocytes were observed in both inoculated calves. BHV-4 was demonstrated in seven samples from the 12 different parts of the nervous system tested from each calf (29.1%), from the cornea, from lymph nodes near to the inoculation site, from the gallbladder and from the bone marrow. Thus a member of the predominantly lymphotropic Gammaherpesvirinae subfamily was detected in neural tissue and other organs that have never been associated with persistence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Bone Marrow / virology
  • Cattle
  • Cattle Diseases / virology*
  • Cornea / virology
  • DNA, Viral / analysis
  • Gallbladder / virology
  • Herpesviridae / genetics
  • Herpesviridae / physiology*
  • Herpesviridae Infections / veterinary*
  • Herpesviridae Infections / virology
  • Lymph Nodes / virology
  • Male
  • Nervous System / virology
  • Polymerase Chain Reaction / veterinary
  • Virus Latency / physiology*


  • DNA, Viral