Rodents, particularly rats, are widely held to be the source of most human cases of leptospirosis. Feral rats were trapped at sites throughout Barbados during two six month surveys: from October to March 1986/87 and from October to March 1994/95. During the first survey, 63 rats were trapped, of which 26 (41%) were identified as Rattus rattus and 37 (59%) as Rattus norvegicus. In the second study, 100 rats were trapped, of which R. rattus comprised 24% (24) and R. norvegicus 76% (76). Cultures of blood, urine and kidney were made in EMJH medium. Leptospires were isolated from 12/63 (19%) and from 16/100 (16%) of the rats during 1986/87 and 1994/95, respectively; 27/28 isolates were recovered from the kidneys or urine or both, while only one isolate was recovered from the blood. During the first study, isolates were identified as serovars copenhageni (11) and arborea (1), while in the second study, serovars copenhageni (9), arborea (5) and bim (1) were identified; one isolate was lost before it could be identified. In the first study, antibodies were detected by microscopic agglutination at a titre of > or = 100 in 26/62 (42%) of rats tested, while in the second survey, 5/100 (5%) of rats had similar titres. In two surveys, conducted eight years apart, we confirmed that rats in Barbados are commonly infected with leptospires, and that viable organisms are found in the kidneys and urine, evidence of chronic infection and thus excretion of leptospires in rodent urine. Moreover, the predominant serovar isolated was copenhageni, of which Rattus spp. are the worldwide reservoir. There was little evidence that rats act as a reservoir for the serovar bim, the most common cause of human leptospirosis in Barbados.