Immunohistochemical detection of E2F-1 in non-Hodgkin's lymphomas: a survey of 124 cases

Mod Pathol. 1998 May;11(5):457-63.


E2F-1 is a transcription factor that mediates cell cycle progression from the G1 to S phase and is normally regulated by a group of proteins, including cyclin D1. Although deregulation of E2F-1 is implicated in neoplastic transformation, in situ examination of this protein has not been performed to date. Using an immunohistochemical technique applied to routinely fixed, paraffin-embedded tissue sections, we evaluated E2F-1 expression in reactive lymphoid tissues and in 124 cases of non-Hodgkin's lymphoma (NHL) of various types. In reactive lymphoid tissues, E2F-1 was expressed predominantly by large noncleaved cells in germinal centers and by a small subset of cortical thymocytes. Mantle zones and splenic marginal zones were negative. Among the NHLs, four types had a relatively high percentage (> 20%) of E2F-1-positive cells: mantle cell lymphoma (19 of 19), lymphoblastic lymphoma (5 of 5), small noncleaved cell lymphoma (4 of 6), and hepatosplenic gammadelta T-cell lymphoma (3 of 3). The consistent detection of many E2F-1-positive cells in mantle cell lymphoma is in contrast to other small B-cell NHLs (n = 29), which had relatively few (< 10%) E2F-1-positive cells. This finding suggests that immunohistochemical staining for E2F-1 as a supplement to the existing markers, such as cyclin D1, might be useful in the differential diagnosis of NHLs composed of small B cells.

MeSH terms

  • Carrier Proteins*
  • Cell Cycle Proteins*
  • DNA-Binding Proteins*
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen / metabolism
  • Lymphoid Tissue / metabolism
  • Lymphoid Tissue / pathology
  • Lymphoma, B-Cell / metabolism
  • Lymphoma, B-Cell / pathology
  • Lymphoma, Non-Hodgkin / metabolism*
  • Lymphoma, Non-Hodgkin / pathology
  • Lymphoma, T-Cell / metabolism
  • Lymphoma, T-Cell / pathology
  • Reference Values
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors / metabolism*


  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • Ki-67 Antigen
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors