LAGE-1, a new gene with tumor specificity

Int J Cancer. 1998 Jun 10;76(6):903-8. doi: 10.1002/(sici)1097-0215(19980610)76:6<903::aid-ijc22>;2-1.


Representational difference analysis was used to identify genes that are expressed in a human melanoma cell line and not in normal skin. A cDNA clone that appeared to be specific for tumors was obtained and the corresponding gene was sequenced. This new gene was named LAGE-I. Using a LAGE-I probe to screen a cDNA library from the same melanoma cell line, we identified a closely related gene, which proved to be identical to NY-ESO-I, a gene recently reported to code for an antigen recognized by autologous antibodies in an esophageal squamous cell carcinoma. Gene LAGE-I maps to Xq28. It comprises 3 exons. Alternative splicing produces 2 major transcripts encoding polypeptides of 210 and 180 residues, respectively. Expression of LAGE-I was observed in 25-50% of tumor samples of melanomas, non-small-cell lung carcinomas, bladder, prostate and head and neck cancers. The only normal tissue that expressed the gene was testis. As for MAGE-AI, expression of LAGE-I is induced by deoxy-azacytidine in lymphoblastoid cells, suggesting that tumoral expression is due to demethylation. The expression of LAGE-I is strongly correlated with that of NY-ESO-I. It is also clearly correlated with the expression of MAGE genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Neoplasm*
  • Antigens, Surface
  • Base Sequence
  • Chromosome Mapping
  • Humans
  • Melanoma / genetics
  • Membrane Proteins*
  • Molecular Sequence Data
  • Neoplasms / genetics*
  • Oncogenes*
  • Proteins / genetics*
  • Tumor Cells, Cultured


  • Antigens, Neoplasm
  • Antigens, Surface
  • CTAG1B protein, human
  • CTAG2 protein, human
  • Membrane Proteins
  • Proteins

Associated data

  • GENBANK/AJ003149
  • GENBANK/AJ223040
  • GENBANK/AJ223041
  • GENBANK/AJ223093