Immunocytochemical localization of calmodulin in the vestibular end-organs of the gerbil

J Vestib Res. May-Jun 1998;8(3):209-16.

Abstract

This study demonstrates the presence of calmodulin in the vestibular end-organs of the gerbil by use of immunocytochemistry. Using fluorescence microscopy, calmodulin was localized to the cytoplasm, cuticular plate, and stereocilia of both type I and type II hair cells in the sensory epithelia of the utricle and cristae ampullaris; no label was found in the supporting cells, the dark cells, or the nerve fibers. There was no immunoreactive distinction between the labeling of type I and type II hair cells in the striolar or extrastriolar regions. Thus, immunocytochemical labeling for calmodulin provides a good marker for hair cells in gerbil vestibular epithelium. The presence of calmodulin in the stereocilia was confirmed by immunoelectron microscopy using secondary antibodies coupled to colloidal gold.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calmodulin / analysis*
  • Cytoplasm / chemistry
  • Cytoplasm / ultrastructure
  • Epithelium / chemistry
  • Epithelium / ultrastructure
  • Fluorescent Antibody Technique, Indirect
  • Gerbillinae
  • Hair Cells, Vestibular / chemistry*
  • Hair Cells, Vestibular / ultrastructure
  • Microscopy, Fluorescence
  • Microscopy, Immunoelectron
  • Saccule and Utricle / chemistry
  • Saccule and Utricle / ultrastructure

Substances

  • Calmodulin