Production and characterization of monoclonal antibodies to the catenin p120ctn

Hybridoma. 1998 Apr;17(2):175-83. doi: 10.1089/hyb.1998.17.175.

Abstract

This report describes the generation and characterization of a panel of monoclonal antibodies (MAb) to the catenin p120ctn. p120ctn (formerly p120cas) is a cadherin-binding protein with structural similarity to the classical catenins beta-catenin and plakoglobin. It was originally identified as a prominent Src substrate and subsequently as a substrate for the Platelet Derived Growth Factor (PDGF), Epidermal Growth Factor (EGF), and Colony Stimulating Factor-1 (CSF-1) receptor tyrosine kinases. To facilitate further study of p120 function, we have generated novel MAbs to both the N- and C-terminal ends of p120 and compared them to previously described antibodies to these regions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Blotting, Western
  • Catenins
  • Cell Adhesion Molecules / immunology*
  • Cell Line
  • Cross Reactions
  • Dogs
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Hybridomas / immunology
  • Mice
  • Phosphoproteins / immunology*
  • Precipitin Tests
  • Rats
  • Species Specificity

Substances

  • Antibodies, Monoclonal
  • Catenins
  • Cell Adhesion Molecules
  • Phosphoproteins
  • delta catenin