Simple One-Tube Reverse Transcription-Polymerase Chain Reaction Protocol Containing Anticontamination Procedure for Detection of GB Virus C/hepatitis G Virus RNA

J Virol Methods. 1998 Mar;71(1):1-6. doi: 10.1016/s0166-0934(97)00205-x.

Abstract

A simple one-tube reverse transcription-polymerase chain reaction protocol for detection of GB virus C/hepatitis G virus (GBV-C/HGV) RNA in serum samples containing anticontamination uracil-N-glycosylase procedure was developed. Amplification products were detected in a standard microtiter plate format using a commercial PCR ELISA kit (Boehringer Mannheim, Germany). Complete agreement between the results of a novel method and the previously determined GBV-C/HGV RNA status was obtained on 131 serum samples (76 GBV-C/HGV RNA positive and 55 GBV-C/HGV RNA negative samples). The novel method provides the potential for automated, simple, rapid and accurate routine detection of GBV-C/HGV RNA in diagnostic virological laboratories.

MeSH terms

  • Flaviviridae / genetics
  • Flaviviridae / isolation & purification*
  • Humans
  • Polymerase Chain Reaction / instrumentation
  • Polymerase Chain Reaction / methods*
  • RNA, Viral / isolation & purification*
  • Reproducibility of Results

Substances

  • RNA, Viral