A simple and sensitive method for detecting adenovirus in serum and urine

J Virol Methods. 1998 Mar;71(1):51-6. doi: 10.1016/s0166-0934(97)00189-4.


A method to detect shed virus in patients' serum and urine was developed following Ad5CMV-p53 gene transfer via direct tumor injections. The procedure differs from those reported previously in that it first uses polyethylene glycol to precipitate adenoviral particles from patient serum or urine. Adenoviral DNA is then extracted following proteinase K digestion. Finally, polymerase chain reaction (PCR) amplification followed by Southern blot transfer are employed to enhance the limit of detection to less than ten viral particles. The detection limit in a 0.25-ml sample is five viral particles in serum and one viral particle in urine. This procedure is sensitive, reproducible, and can be completed in less than 2 days.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / isolation & purification*
  • Blood / virology*
  • Blotting, Southern
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Humans
  • Polyethylene Glycols
  • Polymerase Chain Reaction
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Urine / virology*
  • Virus Shedding


  • DNA, Viral
  • Reagent Kits, Diagnostic
  • Polyethylene Glycols