Diagnosis of cytomegalovirus infection in kidney transplant recipients by a quantitative RNA-DNA hybrid capture assay for cytomegalovirus DNA in leukocytes

Eur J Clin Microbiol Infect Dis. 1998 Feb;17(2):124-7. doi: 10.1007/BF01682170.


The clinical value of a new RNA-DNA hybridization assay for quantification of cytomegalovirus (CMV) DNA in leukocytes [Hybrid Capture CMV DNA Assay (HCA); Murex Biotech, UK] was evaluated. The HCA was compared with an assay for CMV pp65 antigen in leukocytes and an in-house CMV polymerase chain reaction PCR (CMV-PCR) on parallel blood samples. The HCA and the CMV-PCR were less sensitive than the CMV pp65 assay, but the positive predictive value of all three methods for CMV disease was 50% or less. However, when quantitation of viral load by HCA and CMV pp65 assay was taken into consideration, both assays were superior to CMV-PCR in predicting CMV disease.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, Viral / blood
  • Cytomegalovirus / isolation & purification*
  • Cytomegalovirus / physiology
  • Cytomegalovirus Infections / diagnosis*
  • DNA, Viral / analysis
  • Evaluation Studies as Topic
  • Humans
  • Kidney Transplantation / adverse effects*
  • Leukocytes / virology
  • Nucleic Acid Hybridization*
  • Phosphoproteins / immunology
  • Polymerase Chain Reaction
  • Predictive Value of Tests
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Viral Matrix Proteins / immunology


  • Antibodies, Monoclonal
  • Antigens, Viral
  • DNA, Viral
  • Phosphoproteins
  • Reagent Kits, Diagnostic
  • Viral Matrix Proteins
  • cytomegalovirus matrix protein 65kDa