Wingless Repression of Drosophila Frizzled 2 Expression Shapes the Wingless Morphogen Gradient in the Wing

Cell. 1998 May 29;93(5):767-77. doi: 10.1016/s0092-8674(00)81438-5.

Abstract

In Drosophila wing imaginal discs, the Wingless (Wg) protein acts as a morphogen, emanating from the dorsal/ventral (D/V) boundary of the disc to directly define cell identities along the D/V axis at short and long range. Here, we show that high levels of a Wg receptor, Drosophila frizzled 2 (Dfz2), stabilize Wg, allowing it to reach cells far from its site of synthesis. Wg signaling represses Dfz2 expression, creating a gradient of decreasing Wg stability moving toward the D/V boundary. This repression of Dfz2 is crucial for the normal shape of Wg morphogen gradient as well as the response of cells to the Wg signal. In contrast to other ligand-receptor relationships where the receptor limits diffusion of the ligand, Dfz2 broadens the range of Wg action by protecting it from degradation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Biological Transport
  • Blotting, Western
  • Body Patterning
  • Cell Communication
  • Drosophila / embryology*
  • Drosophila Proteins*
  • Frizzled Receptors
  • Gene Expression Regulation*
  • Genes, Insect
  • Immunohistochemistry
  • In Situ Hybridization
  • Models, Biological
  • Morphogenesis
  • Proto-Oncogene Proteins / metabolism*
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics
  • Receptors, G-Protein-Coupled
  • Receptors, Neurotransmitter*
  • Tissue Distribution
  • Wings, Animal / embryology*
  • Wnt1 Protein

Substances

  • Drosophila Proteins
  • Frizzled Receptors
  • Proto-Oncogene Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Neurotransmitter
  • Wnt1 Protein
  • fz2 protein, Drosophila
  • wg protein, Drosophila