Abstract
The type 6 phosphodiesterase (PDE-6) from retinal rod photoreceptors is an alpha beta gamma 2 heterotetramer. The alpha- and beta-subunits contain catalytic sites for cGMP hydrolysis, whereas the gamma-subunits (P gamma) serve as a protein inhibitor of the enzyme. P gamma is believed to be expressed only in photoreceptors. Using RT-PCR, we have amplified the complete coding sequence for P gamma from mouse lung RNA. The expression of P gamma in this tissue may be related to its ability to interact the type 5 phosphodiesterase (PDE-5), which is the predominant cGMP binding protein in lung. We therefore suggest that P gamma may have a wider signaling role in mammalian cells than previously appreciated.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
3',5'-Cyclic-GMP Phosphodiesterases / antagonists & inhibitors
-
3',5'-Cyclic-GMP Phosphodiesterases / biosynthesis*
-
3',5'-Cyclic-GMP Phosphodiesterases / genetics
-
3',5'-Cyclic-GMP Phosphodiesterases / metabolism
-
3',5'-Cyclic-GMP Phosphodiesterases / physiology
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Cyclic AMP-Dependent Protein Kinases / metabolism
-
Cyclic Nucleotide Phosphodiesterases, Type 5
-
Cyclic Nucleotide Phosphodiesterases, Type 6
-
Enzyme Activation / drug effects
-
Lung / enzymology*
-
Mice
-
Molecular Sequence Data
-
RNA, Messenger / analysis
-
Rod Cell Outer Segment / enzymology*
Substances
-
RNA, Messenger
-
Cyclic AMP-Dependent Protein Kinases
-
3',5'-Cyclic-GMP Phosphodiesterases
-
Cyclic Nucleotide Phosphodiesterases, Type 5
-
Cyclic Nucleotide Phosphodiesterases, Type 6
-
Pde5a protein, mouse
-
Pde6b protein, mouse