A serum shock induces circadian gene expression in mammalian tissue culture cells

Cell. 1998 Jun 12;93(6):929-37. doi: 10.1016/s0092-8674(00)81199-x.


The treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals. Oscillating genes include rper1 and rper2 (rat homologs of the Drosophila clock gene period), and the genes encoding the transcription factors Rev-Erb alpha, DBP, and TEF. In rat-1 fibroblasts, up to three consecutive daily oscillations with an average period length of 22.5 hr could be recorded. The temporal sequence of the various mRNA accumulation cycles is the same in cultured cells and in vivo. The serum shock of rat-1 fibroblasts also results in a transient stimulation of c-fos and rper expression and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Clocks / genetics
  • Blood*
  • Carcinoma, Hepatocellular
  • Cell Cycle Proteins
  • Cell Line
  • Circadian Rhythm / genetics*
  • Cycloheximide / pharmacology
  • Fibroblasts / metabolism*
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Genes, Immediate-Early / genetics
  • Genes, fos / genetics
  • Liver / metabolism
  • Nuclear Proteins / genetics
  • Period Circadian Proteins
  • Protein Synthesis Inhibitors / pharmacology
  • RNA, Messenger / biosynthesis
  • RNA, Neoplasm / biosynthesis
  • Rats
  • Transcription Factors / genetics
  • Tumor Cells, Cultured


  • Cell Cycle Proteins
  • Nuclear Proteins
  • Per1 protein, mouse
  • Per1 protein, rat
  • Per2 protein, mouse
  • Per2 protein, rat
  • Period Circadian Proteins
  • Protein Synthesis Inhibitors
  • RNA, Messenger
  • RNA, Neoplasm
  • Transcription Factors
  • Cycloheximide