Endothelial cells differentially express functional CXC-chemokine receptor-4 (CXCR-4/fusin) under the control of autocrine activity and exogenous cytokines

Biochem Biophys Res Commun. 1998 Jun 9;247(1):38-45. doi: 10.1006/bbrc.1998.8499.


Analysis of endothelial cell (EC) chemokine receptor expression by RT-PCR revealed that EC essentially do not express CC-chemokine receptors whereas they express all known CXC-chemokine receptors. Endotheliotropic functions of ligands for CXCR-1, CXCR-2, and CXCR-3 have previously been described. We have consequently performed a detailed analysis of endothelial CXCR-4 expression. CXCR-4 is constitutively expressed by quiescent, resting EC. Cytokine stimulation revealed that bFGF upregulates endothelial CXCR-4 expression, whereas TNF alpha downregulates endothelial CXCR-4 expression. Expression of CXCR-4 mRNA as well as protein is also upregulated in autocrine activated, migrating bovine aortic endothelial cells (BAEC). Furthermore, migrating BAEC preferentially present CXCR-4 on the cell surface as evidenced by cytochemistry and FACS analysis. Lastly, the monospecific CXCR-4 ligand SDF-1 was found to act as a potent inducer of EC chemotaxis. In summary, the data indicate that the CXCR-4/SDF-1 receptor ligand interaction may be an important regulator of activated endothelial cell functions as they occur during vascular remodeling and angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta, Thoracic
  • Cattle
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Chemokine CXCL12
  • Chemokines, CXC / pharmacology
  • Chemotaxis / drug effects
  • Cytokines / pharmacology*
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / physiology
  • Gene Expression Regulation
  • Humans
  • Polymerase Chain Reaction
  • Receptors, CXCR4 / biosynthesis*
  • Receptors, CXCR4 / drug effects
  • Receptors, CXCR4 / genetics
  • Receptors, CXCR4 / physiology
  • Transcription, Genetic
  • Umbilical Veins


  • CXCL12 protein, human
  • Chemokine CXCL12
  • Chemokines, CXC
  • Cytokines
  • Receptors, CXCR4