Cloning and characterization of human estrogen receptor beta isoforms

Biochem Biophys Res Commun. 1998 Jun 9;247(1):75-8. doi: 10.1006/bbrc.1998.8738.


Multiple transcripts which arise from the human estrogen receptor beta (ER beta) gene have been characterized. Three full length isoforms of the hER beta gene, designated hER beta 1-3, were identified in a testis cDNA library. An additional two isoforms, designated hER beta 4 and hER beta 5, were identified by PCR amplification from testis cDNA and from the MDA-MB 435 cell line. hER beta 1 corresponds to the previously described hER beta. All five isoforms diverge at a common position within the predicted helix 10 of the ligand binding domain of hER beta, with nucleotide sequences consistent with differential exon usage. The hER beta isoform mRNAs displayed a differential pattern of expression in human tissues and in tumor cell lines when analyzed by RT-PCR. Further characterization of the three full length isoforms, hER beta 1-3, by in vitro band shift studies indicated that the isoforms were able to form DNA-binding homodimers and heterodimers with each other and with the ER alpha subtype.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA-Binding Proteins / metabolism
  • Dimerization
  • Estrogen Receptor beta
  • Estrogens / genetics
  • Gene Expression
  • Humans
  • Isomerism
  • Male
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • RNA, Messenger / biosynthesis
  • Receptors, Estrogen / biosynthesis
  • Receptors, Estrogen / chemistry*
  • Receptors, Estrogen / genetics*
  • Regulatory Sequences, Nucleic Acid
  • Testis


  • DNA-Binding Proteins
  • Estrogen Receptor beta
  • Estrogens
  • RNA, Messenger
  • Receptors, Estrogen

Associated data

  • GENBANK/AF051427
  • GENBANK/AF061054
  • GENBANK/AF061055