Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans

Yeast. 1998 May;14(7):681-6. doi: 10.1002/(SICI)1097-0061(199805)14:7<681::AID-YEA256>3.0.CO;2-8.

Abstract

A previously isolated partial cDNA encoding a cell wall protein antigen found on hyphal surfaces of the opportunistic fungal pathogen, Candida albicans (Staab et al., 1996) was used to clone the complete hyphal wall protein 1 gene (HWP1). Hyphal forms of C. albicans invade mucosal surfaces of immunocompromised patients such as those with AIDS. HWP1 consisted of an open reading frame predicting an acidic protein (pI of 3.37) with a calculated molecular size of 61,122. The antigenic domain was located in the N-terminal third of the protein. The remainder of the protein contained abundant hydroxy amino acids, and terminated with a string of 15 amino acids typical of sequences specifying post-translational modification with glycosylphosphatidylinositol (6PI). The analyses suggested that Hwp1 is a glucan-linked protein with serine/threonine-rich regions that are predicted to function in extending a ligand-binding domain into the extracellular space.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / chemistry
  • Base Sequence
  • Candida albicans / chemistry
  • Candida albicans / genetics*
  • Candida albicans / growth & development
  • Cell Wall / chemistry
  • Cloning, Molecular
  • DNA, Complementary
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Gene Dosage
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Transcription, Genetic

Substances

  • Amino Acids
  • DNA, Complementary
  • Fungal Proteins

Associated data

  • GENBANK/U64206