Abstract
A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antiporters / genetics*
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Bacillus subtilis / drug effects
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Bacillus subtilis / enzymology*
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Bacillus subtilis / genetics*
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Bacterial Proteins / genetics*
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Base Sequence
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Chromosome Mapping
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Chromosomes, Bacterial
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Gene Deletion*
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Molecular Sequence Data
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Point Mutation*
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Polyribonucleotide Nucleotidyltransferase / genetics*
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Promoter Regions, Genetic
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Tetracycline / pharmacology*
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Tetracycline Resistance / genetics*
Substances
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Antiporters
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Bacterial Proteins
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tetA protein, Bacteria
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Polyribonucleotide Nucleotidyltransferase
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Tetracycline