Abstract
Four cucurbitacin glycosides were isolated from Picriafel-terrae and identified by MS and NMR spectroscopy as picfeltarraenin IA (1), picfeltarraenin IB (2), picfeltarraenin IV (4), and a new compound picfeltarraenin VI (3) (picfeltarraegenin I 3-O-beta-D-xylopyranoside). All four compounds acted as inhibitors on both the classical and alternative pathways of the complement system, with compound 3 exhibiting the highest inhibitory activity (IC50 29 +/- 2 microM and 21 +/- 1 microM, respectively). Compounds 1-4 showed no antiviral, antibacterial, or antifungal activities. Picfeltarraenin IA and IB were tested in an in vitro human tumor cell line panel, but displayed no cytotoxic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents / isolation & purification
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Anti-Infective Agents / pharmacology
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China
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Complement Inactivator Proteins / isolation & purification*
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Complement Inactivator Proteins / pharmacology*
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Complement Pathway, Alternative / drug effects
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Complement Pathway, Classical / drug effects
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Drug Screening Assays, Antitumor
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology
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Humans
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Magnetic Resonance Spectroscopy
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Microbial Sensitivity Tests
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Plants, Medicinal / chemistry*
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Spectrometry, Mass, Fast Atom Bombardment
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
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Tumor Cells, Cultured
Substances
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Anti-Infective Agents
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Complement Inactivator Proteins
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Glycosides