Ruthenium red reduces the Ca2+ sensitivity of Ca2+ uptake into cardiac sarcoplasmic reticulum

Pflugers Arch. 1998 Aug;436(3):338-42. doi: 10.1007/s004240050641.


Ruthenium red inhibits mitochondrial Ca2+ uptake and is widely used as an inhibitor of ryanodine-sensitive Ca2+ channels that function to release Ca2+ from the sarcoplasmic reticulum (SR) of muscle cells. It also has effects on other Ca2+ channels and ion transporters. To study the effects of ruthenium red on Ca2+ transport into the SR of cardiac muscle cells, fluorescence measurements of Ca2+ uptake into cardiac SR vesicles were made. Ruthenium red significantly decreased the Ca2+ sensitivity of SR uptake in a dose-dependent manner at concentrations ranging from 5 microM to 20 microM. There were no significant effects of ruthenium red on the maximum velocity or the Hill coefficient of SR Ca2+ uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Calcium / metabolism*
  • Dogs
  • In Vitro Techniques
  • Myocardium / metabolism*
  • Ruthenium Red / pharmacology*
  • Sarcoplasmic Reticulum / drug effects*
  • Sarcoplasmic Reticulum / metabolism
  • Sarcoplasmic Reticulum / ultrastructure
  • Spectrometry, Fluorescence


  • Ruthenium Red
  • Calcium