Detection of negative allosteric effects between monoclonal antibodies by using an antigenic model-builder computer program

Comput Biol Med. 1998 Jan;28(1):61-73. doi: 10.1016/s0010-4825(97)00042-5.

Abstract

The ability of monoclonal antibodies (MAb) to bind or not simultaneously to the antigen (Ag) is used to establish antigenic maps considering that two different MAb do not bind to the Ag when the corresponding epitopes are overlapped (steric effect). Nevertheless, MAb inducing negative allosteric effect on the Ag could prevent the binding of the second MAb even if it is directed to a separate epitope. We report here that a knowledge-based expert module included in our previously described antigenic model-builder program (MAPAG) was able to differentiate between steric and negative allosteric effects between some MAb.

MeSH terms

  • Algorithms
  • Allosteric Site / immunology*
  • Antibodies, Monoclonal / immunology*
  • Antigen-Antibody Complex / immunology
  • Artificial Intelligence
  • Binding Sites, Antibody / immunology*
  • Computer Simulation*
  • Epitopes / immunology*
  • Expert Systems
  • Humans
  • Image Processing, Computer-Assisted
  • Models, Immunological
  • Software*

Substances

  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Epitopes