Identification and characterisation of an RPD3 homologue from maize (Zea mays L.) that is able to complement an rpd3 null mutant of Saccharomyces cerevisiae

Mol Gen Genet. 1998 May;258(3):288-96. doi: 10.1007/s004380050733.

Abstract

In mammals, yeast and Drosophila, the histone deacetylase RPD3 proteins can alter the expression of genes involved in fundamental biological processes by affecting the degree of acetylation of histones and changing chromatin structure. Here we report the isolation of a cDNA sequence encoding an RPD3 homologue from maize, which is able to complement the phenotype of an rpd3 null mutant of the yeast Saccharomyces cerevisiae. The expression of the corresponding gene(s) was assessed in different maize tissues. The number of homologous loci was estimated by Southern hybridisation to be in the range of two to three, and the chromosomal location of one of these loci was determined. Phylogenetic analysis and tests for relative divergence rates, using related RPD3 sequences from different species, were performed, and suggest that different polymorphic forms of RPD3-like proteins that evolve at distinct rates are present in the species considered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Drosophila Proteins
  • Fungal Proteins / genetics
  • Genes, Reporter / genetics
  • Genetic Complementation Test
  • Histone Deacetylase 1
  • Histone Deacetylases / chemistry*
  • Mutation / genetics
  • Phylogeny
  • Plant Proteins / genetics
  • RNA, Messenger / analysis
  • Repressor Proteins
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Transcription Factors / chemistry*
  • Zea mays / enzymology*

Substances

  • Drosophila Proteins
  • Fungal Proteins
  • Plant Proteins
  • RNA, Messenger
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • RPD3 protein, S cerevisiae
  • HDAC1 protein, Drosophila
  • Histone Deacetylase 1
  • Histone Deacetylases

Associated data

  • GENBANK/AF035815