Antibody testing and RT-PCR results in hepatitis C virus (HCV) infection: HCV-RNA detection in PBMC of plasma viremia-negative HCV-seropositive persons

Infection. May-Jun 1998;26(3):151-4. doi: 10.1007/BF02771840.


To evaluate the concordance between viremia and antibody testing in hepatitis C virus (HCV) diagnosis, 682 serum or plasma samples collected from patients with known or suspected HCV infection were tested. An overall concordance of 77% between serological and PCR results was found, 5% was RNA positive/antibody negative and 18% antibody positive/RNA negative. The relationship between HCV infection, risk group and clinical diagnosis was studied in 116 patients: the presence of anti-HCV antibody without viremia was shown in 72.7% of asymptomatic subjects and 17.6% of chronic hepatitis subjects without interferon treatment. However, the detection of HCV-RNA in peripheral blood mononuclear cells (PBMC) in four out of 38 plasma viremia-negative HCV-seropositive subjects (10.5%), showed that HCV-RNA could persist in PBMC and could begin the viral replication again at different times. The detection of HCV-RNA in PBMC in anti-HCV-positive subjects without viremia could reduce false-negative results of HCV-RNA testing by RT-PCR in serum or plasma.

Publication types

  • Comparative Study

MeSH terms

  • DNA Primers
  • False Negative Reactions
  • Hepacivirus / genetics
  • Hepacivirus / immunology
  • Hepacivirus / isolation & purification*
  • Hepatitis C / diagnosis*
  • Hepatitis C / immunology
  • Hepatitis C Antibodies / blood*
  • Humans
  • Leukocytes, Mononuclear / immunology
  • Polymerase Chain Reaction / methods
  • Predictive Value of Tests
  • RNA, Viral / blood*
  • RNA-Directed DNA Polymerase
  • Risk
  • Viremia / diagnosis*
  • Viremia / virology*


  • DNA Primers
  • Hepatitis C Antibodies
  • RNA, Viral
  • RNA-Directed DNA Polymerase