Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)

Biochem Biophys Res Commun. 1998 Jun 29;247(3):905-9. doi: 10.1006/bbrc.1998.8895.


The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / physiology
  • Binding, Competitive
  • Chondrocytes / metabolism*
  • Connective Tissue Growth Factor
  • Cross-Linking Reagents / metabolism
  • Growth Substances / metabolism*
  • Humans
  • Immediate-Early Proteins*
  • Intercellular Signaling Peptides and Proteins*
  • Iodine Radioisotopes / metabolism
  • Precipitin Tests
  • Protein Binding
  • Receptors, Cell Surface / chemistry*
  • Recombinant Proteins / metabolism
  • Tumor Cells, Cultured


  • CCN2 protein, human
  • Cross-Linking Reagents
  • Growth Substances
  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Iodine Radioisotopes
  • Receptors, Cell Surface
  • Recombinant Proteins
  • Connective Tissue Growth Factor