Gene cloning and characterization of a novel cellulose-binding beta-glucosidase from Phanerochaete chrysosporium

Appl Environ Microbiol. 1998 Jul;64(7):2748-54. doi: 10.1128/AEM.64.7.2748-2754.1998.

Abstract

Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular beta-glucosidase (CBGL) from Phanerochaete chrysosporium suggested that CBGL is organized into two domains, an N-terminal cellulose-binding domain and a C-terminal catalytic domain. Genomic sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated that CBGL is encoded by two alleles, cbgl-1 and cbgl-2, of a single gene.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Basidiomycota / enzymology*
  • Basidiomycota / genetics
  • Fungal Proteins / genetics*
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • beta-Glucosidase / genetics*

Substances

  • Fungal Proteins
  • beta-Glucosidase

Associated data

  • GENBANK/AF036872
  • GENBANK/AF036873