cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme

Biosci Biotechnol Biochem. 1998 May;62(5):1037-40. doi: 10.1271/bbb.62.1037.


A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • Cross-Linking Reagents / metabolism
  • DNA Primers
  • Gene Expression Regulation, Enzymologic*
  • Humans
  • Molecular Sequence Data
  • Phosphotransferases (Alcohol Group Acceptor) / biosynthesis
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / physiology*
  • Sulfate Adenylyltransferase / biosynthesis
  • Sulfate Adenylyltransferase / genetics
  • Sulfate Adenylyltransferase / physiology*


  • Cross-Linking Reagents
  • DNA Primers
  • Phosphotransferases (Alcohol Group Acceptor)
  • adenylylsulfate kinase
  • Sulfate Adenylyltransferase

Associated data

  • GENBANK/AF033026