Molecular cloning and DNA sequencing of the Staphylococcus aureus UDP-N-acetylmuramyl tripeptide synthetase (murE) gene, essential for the optimal expression of methicillin resistance

Microb Drug Resist. 1998 Summer;4(2):85-90. doi: 10.1089/mdr.1998.4.85.

Abstract

The Tn551 insertion in mutant RUSA235 of a highly methicillin resistant Staphylococcus aureus strain results in drastic reduction in the level of methicillin resistance and abnormalities, both in the composition of the peptidoglycan and of the cell wall precursor pool. Cloning and sequencing of the inactivated gene indicates that it is the murE gene of Staphylococcus aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteriophage lambda / genetics
  • Cloning, Molecular*
  • DNA Transposable Elements / genetics
  • Deoxyribonuclease EcoRI
  • Genetic Vectors
  • Humans
  • Methicillin Resistance / genetics*
  • Molecular Sequence Data
  • Peptide Synthases / genetics*
  • Plasmids / genetics
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Analysis, DNA*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / genetics*

Substances

  • DNA Transposable Elements
  • Deoxyribonuclease EcoRI
  • Peptide Synthases
  • UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase