Characterization and targeted disruption of a glycosyltransferase gene in the tylosin producer, Streptomyces fradiae

Gene. 1998 Jul 3;214(1-2):95-100. doi: 10.1016/s0378-1119(98)00210-8.


An open reading frame, designated tylN, has been identified by sequence analysis at one end of the tylosin biosynthetic gene cluster of Streptomyces fradiae, alongside a cluster of genes encoding the biosynthesis of dTDP-deoxyallose. This 6-deoxyhexose sugar is converted to mycinose, via bis O-methylation, following attachment to the polyketide lactone during tylosin biosynthesis. The deduced product of tylN is similar to several glycosyltransferases, authentic and putative, and displays a consensus sequence motif that appears to be characteristic of a sub-group of such enzymes. Specific disruption of tylN within the S. fradiae genome resulted in the production of demycinosyl-tylosin, whereas other glycosyltransferase activities involved in tylosin biosynthesis were not affected. Evidently, tylN encodes deoxyallosyl transferase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Bacterial / genetics
  • Fermentation
  • Gene Targeting
  • Genes, Bacterial*
  • Glycosyltransferases / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Open Reading Frames
  • Sequence Homology, Amino Acid
  • Streptomyces / enzymology
  • Streptomyces / genetics*
  • Streptomyces / metabolism*
  • Tylosin / analogs & derivatives
  • Tylosin / biosynthesis*


  • DNA, Bacterial
  • Glycosyltransferases
  • Tylosin

Associated data

  • GENBANK/AJ005397