Epstein-Barr virus-induced molecule 1 ligand chemokine is expressed by dendritic cells in lymphoid tissues and strongly attracts naive T cells and activated B cells

J Exp Med. 1998 Jul 6;188(1):181-91. doi: 10.1084/jem.188.1.181.


Movement of T and B lymphocytes through secondary lymphoid tissues is likely to involve multiple cues that help the cells navigate to appropriate compartments. Epstein-Barr virus- induced molecule 1 (EBI-1) ligand chemokine (ELC/MIP3beta) is expressed constitutively within lymphoid tissues and may act as such a guidance cue. Here, we have isolated mouse ELC and characterized its expression pattern and chemotactic properties. ELC is expressed constitutively in dendritic cells within the T cell zone of secondary lymphoid tissues. Recombinant ELC was strongly chemotactic for naive (L-selectinhi) CD4 T cells and for CD8 T cells and weakly attractive for resting B cells and memory (L-selectinlo) CD4 T cells. After activation through the B cell receptor, the chemotactic response of B cells was enhanced. Like its human counterpart, murine ELC stimulated cells transfected with EBI-1/CC chemokine receptor 7 (CCR7). Our findings suggest a central role for ELC in promoting encounters between recirculating T cells and dendritic cells and in the migration of activated B cells into the T zone of secondary lymphoid tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism*
  • Calcium / metabolism
  • Cell Movement / physiology
  • Chemokine CCL19
  • Chemokines, CC / physiology*
  • Chemotaxis / physiology
  • Dendritic Cells / metabolism
  • Flow Cytometry
  • Gene Expression Regulation / genetics
  • Lymph Nodes / cytology
  • Mice
  • Peyer's Patches / cytology
  • RNA, Messenger / metabolism
  • Receptors, CCR7
  • Receptors, Cell Surface / genetics
  • Receptors, Chemokine*
  • Sequence Alignment
  • Spleen / cytology
  • T-Lymphocytes / metabolism*
  • Transfection / genetics


  • CCL19 protein, human
  • CCR7 protein, human
  • Ccl19 protein, mouse
  • Ccr7 protein, mouse
  • Chemokine CCL19
  • Chemokines, CC
  • RNA, Messenger
  • Receptors, CCR7
  • Receptors, Cell Surface
  • Receptors, Chemokine
  • Calcium