High-performance liquid chromatography determination of mycophenolic acid and its glucuronide metabolite in human plasma

J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):229-34. doi: 10.1016/s0378-4347(97)00678-6.


Two HPLC-UV assays are reported here: one is a rapid assay for mycophenolic acid (MPA) and the other is a simultaneous assay for MPA and its metabolite mycophenolic acid glucuronide (MPAG). For both methods, plasma samples (500 microl) with added internal standard were acidified and extracted using C18 solid-phase extraction cartridges. Chromatographic separation was achieved on a C18 Novapak column using a mobile phase consisting of methanol-0.05% orthophosphoric acid (40:60, v/v) for the rapid MPA assay and 30:70 for the simultaneous MPA and MPAG assay. The assays were linear over the ranges 0.1 to 50.0 mg/l for MPA and 2.8 to 225.8 mg/l for MPAG. Mean absolute recovery for all analytes was >99%. These methods are suitable for therapeutic drug monitoring and pharmacokinetic studies.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Glucuronates / blood*
  • Glucuronates / pharmacokinetics
  • Glucuronides
  • Humans
  • Mycophenolic Acid / analogs & derivatives*
  • Mycophenolic Acid / blood*
  • Mycophenolic Acid / pharmacokinetics


  • Glucuronates
  • Glucuronides
  • mycophenolic acid glucuronide
  • Mycophenolic Acid