Sequence and functional characterization of RNase P RNA from the chl alb containing cyanobacterium Prochlorothrix hollandica

FEBS Lett. 1998 May 29;428(3):161-4. doi: 10.1016/s0014-5793(98)00519-5.

Abstract

Only a few complete sequences and very limited functional data are available for the catalytic RNA component of cyanobacterial RNase P. The RNase P RNA from the chl alb containing cyanobacterium Prochlorothrix hollandica belongs to a rarely found structural subtype with an extended P15/16 domain. We have established conditions for optimal in vitro ribozyme activity, and determined the kinetic parameters for cleavage of pre-tRNA(Tyr). Analysis of pre-tRNA mutants revealed that the T-stem sequence only plays a modulating role, whereas the CCA end is essential for efficient product formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Endoribonucleases / chemistry*
  • Endoribonucleases / metabolism*
  • Kinetics
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis
  • Nucleic Acid Conformation*
  • Polymerase Chain Reaction
  • Prochlorothrix / genetics
  • Prochlorothrix / metabolism*
  • RNA Precursors / chemistry
  • RNA Precursors / genetics
  • RNA Precursors / metabolism
  • RNA, Bacterial / chemistry*
  • RNA, Bacterial / metabolism*
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / metabolism*
  • RNA, Transfer, Tyr / chemistry
  • RNA, Transfer, Tyr / genetics
  • RNA, Transfer, Tyr / metabolism
  • Ribonuclease P
  • Sequence Deletion

Substances

  • RNA Precursors
  • RNA, Bacterial
  • RNA, Catalytic
  • RNA, Transfer, Tyr
  • pre-tRNA(Tyr)
  • Endoribonucleases
  • Ribonuclease P

Associated data

  • GENBANK/Y16812