Abstract
Only a few complete sequences and very limited functional data are available for the catalytic RNA component of cyanobacterial RNase P. The RNase P RNA from the chl alb containing cyanobacterium Prochlorothrix hollandica belongs to a rarely found structural subtype with an extended P15/16 domain. We have established conditions for optimal in vitro ribozyme activity, and determined the kinetic parameters for cleavage of pre-tRNA(Tyr). Analysis of pre-tRNA mutants revealed that the T-stem sequence only plays a modulating role, whereas the CCA end is essential for efficient product formation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cloning, Molecular
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Endoribonucleases / chemistry*
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Endoribonucleases / metabolism*
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Kinetics
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Models, Molecular
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Molecular Sequence Data
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Mutagenesis
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Nucleic Acid Conformation*
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Polymerase Chain Reaction
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Prochlorothrix / genetics
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Prochlorothrix / metabolism*
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RNA Precursors / chemistry
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RNA Precursors / genetics
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RNA Precursors / metabolism
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RNA, Bacterial / chemistry*
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RNA, Bacterial / metabolism*
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RNA, Catalytic / chemistry*
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RNA, Catalytic / metabolism*
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RNA, Transfer, Tyr / chemistry
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RNA, Transfer, Tyr / genetics
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RNA, Transfer, Tyr / metabolism
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Ribonuclease P
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Sequence Deletion
Substances
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RNA Precursors
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RNA, Bacterial
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RNA, Catalytic
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RNA, Transfer, Tyr
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pre-tRNA(Tyr)
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Endoribonucleases
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Ribonuclease P