Induction of Rapid T Cell Activation and Tolerance by Systemic Presentation of an Orally Administered Antigen

Immunity. 1998 Jun;8(6):667-73. doi: 10.1016/s1074-7613(00)80571-3.

Abstract

To understand how orally introduced antigen regulates peripheral immune responses, we fed cytochrome c protein to mice transgenic for the beta chain of a cytochrome c-specific TCR and followed the antigen-specific T cell responses with a cyt c/I-Ek tetramer staining reagent. We find that within 6 hr of cytochrome c administration, antigen-specific systemic T cell activation is induced, and spleen cells gain the ability to stimulate cytochrome c-specific T cell responses. Feeding multiple low doses of cytochrome c down-regulates the systemic immune response, which can be correlated with a reduction of antigen-specific T cells and not with immune deviation. These results suggest that systemic distribution of antigen contributes significantly to oral tolerance induction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Oral
  • Animals
  • Antigen Presentation*
  • Antigens / administration & dosage
  • Antigens / immunology*
  • Cytochrome c Group / genetics
  • Cytochrome c Group / immunology
  • Immune Tolerance*
  • Lymphocyte Activation / immunology*
  • Mice
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • T-Lymphocytes / immunology*

Substances

  • Antigens
  • Cytochrome c Group
  • Receptors, Antigen, T-Cell, alpha-beta