Virus-specific CD8+ T cells in primary and secondary influenza pneumonia

Immunity. 1998 Jun;8(6):683-91. doi: 10.1016/s1074-7613(00)80573-7.


Virus-specific CD8+ effector T cells (eCTL) are enriched in the lungs of mice with primary influenza pneumonia, though later detection of memory T cells (mCTL) in the mediastinal lymph nodes (MLN) or spleen by peptide-based staining protocols is at the limits of flow cytometric analysis. Respiratory challenge with an H3N2 virus months after H1N1 priming induces a massive recall response, which reduces virus titers 2-3 days earlier than in nave controls. Influenza-specific mCTL produce interferon-gamma within 6 hr, but still take 4-5 days to localize to the infected respiratory tract. The delay reflects that the recall response develops first in the MLN, which contains relatively few mCTL. The response to a subdominant epitope is less obvious after secondary challenge.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen Presentation
  • Antigens, Viral / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Cytotoxicity, Immunologic*
  • Mice
  • Mice, Inbred C57BL
  • Orthomyxoviridae / immunology*
  • Orthomyxoviridae Infections / immunology*
  • Pneumonia, Viral / immunology*


  • Antigens, Viral