Association of DAP12 with activating CD94/NKG2C NK cell receptors

Immunity. 1998 Jun;8(6):693-701. doi: 10.1016/s1074-7613(00)80574-9.

Abstract

While the inhibitory NK cell receptors for MHC class I express immunoreceptor tyrosine-based inhibitory motifs that recruit intracellular tyrosine phosphatases and prevent NK cell effector function, the activating NK cell receptors lack intrinsic sequences required for cellular stimulation. CD94/NKG2C, an activating NK cell receptor of the C-type lectin superfamily that binds to HLA-E, noncovalently associates with DAP12, a membrane receptor containing an immunoreceptor tyrosine-based activating motif. Efficient expression of CD94/NKG2C on the cell surface requires the presence of DAP12, and charged residues in the transmembrane domains of DAP12 and NKG2C are necessary for this interaction. These results provide a molecular basis for the assembly of NK cell receptors for MHC class I involved in cellular activation and inhibition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Antigens, CD / immunology*
  • Cell Line
  • HLA Antigens / immunology*
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Killer Cells, Natural / immunology*
  • Lectins / immunology
  • Lectins, C-Type*
  • Lymphocyte Activation*
  • Membrane Glycoproteins / immunology*
  • Membrane Proteins
  • Molecular Sequence Data
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Immunologic / immunology*
  • Transfection

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, CD
  • HLA Antigens
  • HLA-E antigen
  • Histocompatibility Antigens Class I
  • KLRD1 protein, human
  • Lectins
  • Lectins, C-Type
  • Membrane Glycoproteins
  • Membrane Proteins
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Immunologic
  • TYROBP protein, human