In vivo neutralization of P-selectin inhibits leukocyte-endothelial interactions in retinal microcirculation during ocular inflammation

Microvasc Res. 1998 May;55(3):230-40. doi: 10.1006/mvre.1998.2084.


P-selectin is one of the adhesion molecules involved in leukocyte rolling during an inflammatory reaction. The aim of this study was to examine the role of P-selectin in leukocyte-endothelial interactions in retinal microcirculation during ocular inflammation, known as endotoxin-induced uveitis (EIU), in vivo. EIU was induced in Lewis rats by footpad injection of lipopolysaccharide (LPS). At the time of LPS treatment or 12 h later, anti-rat P-selectin mAb (ARP) was injected intravenously, and its effect on leukocyte behavior in the retina was studied after intravital staining with acridine orange using a scanning laser ophthalmoscope. P-selectin gene expression in the retina was also studied by a semiquantitative polymerase chain reaction (PCR) method. Administration of ARP at the time of LPS treatment significantly reduced the number of rolling leukocytes at 6 and 12 h by 68% (P < 0.05) and 83% (P < 0.01), respectively, and the number of cells infiltrating the vitreous at 48 h by 61% (P < 0.05). Interestingly, ARP significantly inhibited the vasodilation observed during EIU. In contrast, delayed administration of ARP blocked neither cellular infiltration nor vasodilation. P-selectin gene expression was upregulated during the course of EIU. In conclusion, P-selectin may significantly contribute to the development of inflammation in the early stage of endotoxin-induced ocular inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Blocking / pharmacology
  • Antibodies, Monoclonal / pharmacology
  • Cell Communication* / immunology*
  • Cell Movement / immunology
  • Endothelium, Vascular / pathology*
  • Endothelium, Vascular / physiopathology
  • Female
  • Leukocyte Count
  • Leukocytes / pathology*
  • Microcirculation / pathology
  • Microcirculation / physiopathology
  • P-Selectin / immunology*
  • P-Selectin / physiology*
  • Rats
  • Rats, Inbred Lew
  • Retinitis / pathology*
  • Retinitis / physiopathology
  • Uveitis / pathology*
  • Uveitis / physiopathology


  • Antibodies, Blocking
  • Antibodies, Monoclonal
  • P-Selectin