A Family of Flavoproteins in the Domains Archaea and Bacteria

Eur J Biochem. 1998 Jun 1;254(2):325-32. doi: 10.1046/j.1432-1327.1998.2540325.x.

Abstract

A family of flavoproteins, called A-type flavoproteins, is described. It consists of 14 protein sequences of 385-597 amino acids in length, 7 from methanogens (domain: Archaea), 5 from phototrophic prokaryotes, one from Escherichia coli, and a partial sequence from the sulfate reducer Desulfovibrio gigas (domain: Bacteria). No similar sequence could be found in the domain Eucarya. All sequences show significant similarity over a 385-400 amino acid portion overlapping a recognizable flavodoxin signature starting at positions 245-285 of the common core sequence. Cofactor analysis and, to some extent, analysis of the primary structure of six A-type flavoproteins, three of which are structurally characterized here, support the existence of four sub-families: (a) simple flavoproteins binding only FMN; (b) diflavin flavoproteins binding FMN and FAD; (c) a flavorubredoxin binding FMN and iron; (d) a hemoflavoprotein. The possible involvement of A-type flavoproteins in the metabolism of oxygen, as suggested for D. gigas hemoflavoprotein [Gomes, C. M., Silva, G., Oliveira, S., LeGall, J., Liu, M.-Y., Xavier, A. V., Rodrigues-Pousada, C. & Teixeira, M. (1997) J. Biol. Chem. 272, 22502-22508], is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Archaeal Proteins / chemistry
  • Archaeal Proteins / classification
  • Archaeal Proteins / genetics*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Cyanobacteria / genetics
  • DNA Primers / genetics
  • DNA, Archaeal / genetics
  • DNA, Bacterial / genetics
  • Desulfovibrio / genetics
  • Escherichia coli / genetics
  • Flavoproteins / chemistry
  • Flavoproteins / classification
  • Flavoproteins / genetics*
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / genetics
  • Molecular Sequence Data
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Rhodobacter capsulatus / genetics
  • Rubredoxins / chemistry
  • Rubredoxins / genetics
  • Sequence Homology, Amino Acid

Substances

  • Archaeal Proteins
  • Bacterial Proteins
  • DNA Primers
  • DNA, Archaeal
  • DNA, Bacterial
  • Flavoproteins
  • Recombinant Proteins
  • Rubredoxins
  • Mixed Function Oxygenases
  • nitrilotriacetate monooxygenase