Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80

Mol Cell. 1998 Apr;1(5):685-96. doi: 10.1016/s1097-2765(00)80068-4.


Gametogenesis requires the successful coordination of two key processes, meiotic nuclear division and gamete morphogenesis. A central regulatory step in progression through gametogenesis occurs at the pachytene stage of meiotic prophase. We find that Ndt80 functions at pachytene of yeast gametogenesis (sporulation) to activate transcription of a set of genes required for both meiotic division (e.g., B-type cyclins) and gamete formation (e.g., SPS1). Ectopic synthesis of Ndt80 in vegetative cells induces transcription of these genes, and recombinant Ndt80 protein binds to a conserved sequence in their upstream region. Transcription of NDT80 itself is dependent on Ime1, which activates expression of early sporulation genes. Transcription of the Ndt80-regulated gene CLB1 is mediated by the checkpoint gene RAD17. Thus Ndt80 is a pivotal component of a transcriptional cascade programming yeast gametogenesis and may also be a target of meiotic checkpoint control.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cyclin B / genetics
  • DNA-Binding Proteins*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal / physiology
  • Meiosis / physiology
  • RNA, Fungal / metabolism
  • Recombination, Genetic / physiology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae Proteins*
  • Spores, Fungal / genetics
  • Transcription Factors*
  • Transcription, Genetic / physiology*


  • Cyclin B
  • DNA-Binding Proteins
  • Fungal Proteins
  • NDT80 protein, S cerevisiae
  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors