Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 May;1(6):895-904.
doi: 10.1016/s1097-2765(00)80088-x.

An Activator Target in the RNA Polymerase II Holoenzyme


An Activator Target in the RNA Polymerase II Holoenzyme

S S Koh et al. Mol Cell. .


Expression of protein-coding genes in eukaryotes involves the recruitment, by transcriptional activator proteins, of a transcription initiation apparatus consisting of greater than 50 polypeptides. Recent genetic and biochemical evidence in yeast suggests that a subset of these proteins, called SRB proteins, are likely targets for transcriptional activators. We demonstrate here, through affinity chromatography, photo-cross-linking, and surface plasmon resonance experiments, that the GAL4 activator interacts directly with the SRB4 subunit of the RNA polymerase II holoenzyme. The GAL4 activation domain binds to two essential segments of SRB4. The physiological relevance of this interaction is confirmed by mutations in SRB4, which occur within its GAL4-binding domain and which restore activation in vivo by a GAL4 derivative bearing a mutant activation domain.

Similar articles

See all similar articles

Cited by 81 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources