Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

Nat Biotechnol. 1998 Jul;16(7):642-6. doi: 10.1038/nbt0798-642.


We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Blastocyst / physiology*
  • Cattle / genetics*
  • Cell Differentiation
  • Cell Fusion
  • Chimera*
  • Ectoderm / physiology
  • Embryo Transfer
  • Endoderm / physiology
  • Gene Transfer Techniques
  • Genetic Therapy
  • Mesoderm / physiology
  • Stem Cells / physiology*
  • beta-Galactosidase / analysis


  • beta-Galactosidase