A 4-year study of plasma ochratoxin A in a selected population in Tokyo by immunoassay and immunoaffinity column-linked HPLC

Food Chem Toxicol. 1998 May;36(5):445-9. doi: 10.1016/s0278-6915(98)00004-0.


Employing a competitive ELISA (cELISA) based on monoclonal antibody and a novel immunoaffinity-column (IAC)-linked HPLC-fluorometry, ochratoxin A (OTA) levels in the plasma of 184 healthy volunteers (130 males, 54 females) were surveyed in Tokyo during the 4 years from 1992 to 1996. It was found that 85% of the cases were positive for OTA except 38% in 1994, and an average value in the positives was estimated as 68 pg/ml. This suggests that the population in Tokyo is exposed to OTA at high frequency, although the level in plasma is far less than that reported in Europe and Canada. A partial contribution of fermented dietary foodstuffs, coffee and wine is suspected as the source of the OTA observed in the human plasma.

MeSH terms

  • Chromatography, Affinity / methods
  • Chromatography, High Pressure Liquid
  • Environmental Exposure*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Food Contamination
  • Humans
  • Immunoassay / methods
  • Male
  • Mycotoxins / blood*
  • Ochratoxins / blood*
  • Tokyo


  • Mycotoxins
  • Ochratoxins
  • ochratoxin A