Cloning of the V-ATPase B subunit cDNA from Culex quinquefasciatus and expression of the B and C subunits in mosquitoes

Insect Mol Biol. 1998 Aug;7(3):223-32. doi: 10.1111/j.1365-2583.1998.00069.x.

Abstract

The V-ATPase B subunit cDNA isolated from the midgut and Malpighian tubules of Culex quinquefasciatus larvae has a 1476 bp ORF encoding a 492 amino acids protein with a predicted mass of 54.8 kDa. Northern blot analysis reveals the presence of 1.8 and 4.2 kb transcripts in larvae and a 3.0 kb transcript in pupae. A single 57 kDa protein band is detected in immunoblot analysis of protein extracts from C. quinquefasciatus and A. aegypti larvae. Using antibodies to the B subunits we demonstrate high-level expression of these subunits in ion-transporting cells of caeca and anterior midgut, and in Malpighian tubules and rectum. High V-ATPase expression was also observed in the larval salivary glands, central nervous system neurophile, the thoracic endocrine complex and in imaginal discs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aedes
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Cloning, Molecular
  • Culex / enzymology*
  • Culex / genetics
  • DNA, Complementary
  • Gene Expression
  • Molecular Sequence Data
  • Proton-Translocating ATPases / genetics*
  • Rabbits
  • Vacuolar Proton-Translocating ATPases*

Substances

  • DNA, Complementary
  • Vacuolar Proton-Translocating ATPases
  • Proton-Translocating ATPases

Associated data

  • GENBANK/AF037468