Repeated tertiary fold of RNA polymerase II and implications for DNA binding

J Mol Biol. 1998 Jul 17;280(3):317-22. doi: 10.1006/jmbi.1998.1557.

Abstract

X-ray diffraction data from two forms of yeast RNA polymerase II crystals indicate that the two largest subunits of the polymerase, Rpb1 and Rpb2, may have similar folds, as is suggested by secondary structure predictions. DNA may bind between the two subunits with its 2-fold axis aligned to a pseudo 2-fold axis of the protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • DNA / metabolism*
  • Fungal Proteins / chemistry*
  • Fungal Proteins / metabolism
  • Molecular Sequence Data
  • Protein Folding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary*
  • RNA Polymerase II / chemistry*
  • RNA Polymerase II / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • X-Ray Diffraction
  • Yeasts / enzymology

Substances

  • Fungal Proteins
  • DNA
  • RNA Polymerase II