Assignment of immunoglobulin G1 and G2 concentrations to pneumococcal capsular polysaccharides 3, 6B, 14, 19F, and 23F in pneumococcal reference serum 89-SF

Clin Diagn Lab Immunol. 1998 Jul;5(4):561-6.

Abstract

We describe standardization of an enzyme-linked immunosorbent assay (ELISA) for measuring immunoglobulin G1 (IgG1) and IgG2 concentrations of antibodies to pneumococcal capsular polysaccharide (Pnc PS). The ELISA uses a human pneumococcal reference serum pool, lot 89-SF, as a reference. IgG1 and IgG2 concentrations were assigned to antibodies to Pnc PS serotypes 3, 6B, 14, 19F, and 23F in 89-SF by ELISA using affinity-purified monoisotypic IgG1 and IgG2 preparations. The sum of IgG1 and IgG2 concentrations in 89-SF agrees well with the previously assigned IgG concentrations. The IgG1 and IgG2 values in 89-SF were used to measure antibodies to Pnc PS 6B, 14, and 23F in adult pre- and postimmunization sera and the sum of IgG1 and IgG2 concentrations correlated well with the IgG values. Furthermore, the IgG2/IgG1 ratio did not affect the detection of IgG1, the isotype usually represented by a lower concentration.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial
  • Bacterial Vaccines / administration & dosage
  • Binding, Competitive
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / standards*
  • Evaluation Studies as Topic
  • Humans
  • Immunoglobulin G / blood*
  • Polysaccharides, Bacterial / immunology*
  • Reference Standards
  • Streptococcus pneumoniae / immunology*
  • Vaccines, Conjugate / administration & dosage

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Vaccines
  • Immunoglobulin G
  • Polysaccharides, Bacterial
  • Vaccines, Conjugate