Identification of a rough strain of Escherichia coli O157:H7 that produces no detectable O157 antigen

P Feng; R C Sandlin; C H Park; R A Wilson; M Nishibuchi

doi:10.1128/JCM.36.8.2339-2341.1998

Identification of a rough strain of Escherichia coli O157:H7 that produces no detectable O157 antigen

J Clin Microbiol. 1998 Aug;36(8):2339-41. doi: 10.1128/JCM.36.8.2339-2341.1998.

Authors

P Feng¹, R C Sandlin, C H Park, R A Wilson, M Nishibuchi

Affiliation

¹ Division of Microbiological Studies, Food and Drug Administration, Washington, D.C. 20204, USA. pxf@fdacf.ssw.dhhs.gov

Abstract

MA6, an O157:H7-like strain, did not react with most anti-O157 kits examined; however, it had the rfbE gene that is essential for O157 expression and carried O157:H7 virulence factors. Lipopolysaccharide analysis showed that MA6 is a rough strain that does not produce the O157 antigen, but genetically, it belongs in the O157:H7 clonal group.

MeSH terms

Animals
Bacterial Typing Techniques
Carbohydrate Epimerases / genetics
Cattle
Escherichia coli O157 / classification*
Escherichia coli O157 / immunology*
Escherichia coli O157 / isolation & purification
Lipopolysaccharides / analysis
Meat / microbiology
O Antigens / analysis*
Polymerase Chain Reaction / methods
Serotyping
Transaminases / genetics

Substances

Lipopolysaccharides
O Antigens
Transaminases
Carbohydrate Epimerases
perosamine synthetase