Biochemical and biological analyses of farnesyl-protein transferase inhibitors

N E Kohl; K S Koblan; C A Omer; A Oliff; J B Gibbs

doi:10.1385/0-89603-488-7:283

Biochemical and biological analyses of farnesyl-protein transferase inhibitors

Methods Mol Biol. 1998:84:283-91. doi: 10.1385/0-89603-488-7:283.

Authors

N E Kohl¹, K S Koblan, C A Omer, A Oliff, J B Gibbs

Affiliation

¹ Department of Cancer Research, Merck Research Laboratories, West Point, PA, USA.

PMID: 9666457
DOI: 10.1385/0-89603-488-7:283

Abstract

The methods outlined in Subheading 3. provide a logical sequence of assays with which to evaluate the biochemical and biological properties of potential FPTase inhibitors. The clinical predictability of these assays must await the evaluation of one or more of these compounds in humans.

MeSH terms

Alkyl and Aryl Transferases / antagonists & inhibitors*
Animals
Enzyme Inhibitors / chemistry
Enzyme Inhibitors / pharmacology*
Genes, ras
Humans
Mice
Mice, Nude
Mice, Transgenic
Molecular Biology / methods

Substances

Enzyme Inhibitors
Alkyl and Aryl Transferases
p21(ras) farnesyl-protein transferase