Insulin increases expression of apobec-1, the catalytic subunit of the apolipoprotein B mRNA editing complex in rat hepatocytes

Metabolism. 1998 Jul;47(7):869-73. doi: 10.1016/s0026-0495(98)90128-7.

Abstract

We have previously shown that chronic insulin treatment of rat hepatocytes increases the fraction of edited apolipoprotein B (apoB) mRNA from approximately 50% to as much as 90%. We have now examined the effect of insulin on apobec-1 mRNA abundance and demonstrate that increased editing of apoB mRNA following insulin treatment is accompanied by elevated apobec-1 mRNA levels in primary rat hepatocytes. Time-course measurements of the effects of insulin on apoB mRNA editing and apobec-1 mRNA abundance showed that both were elevated almost maximally within 48 hours and sustained for at least 5 days of insulin treatment.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • APOBEC-1 Deaminase
  • Animals
  • Apolipoproteins B / genetics*
  • Catalysis / drug effects
  • Cells, Cultured
  • Cytidine Deaminase / biosynthesis*
  • Cytidine Deaminase / drug effects
  • Cytidine Deaminase / genetics*
  • Insulin / pharmacology*
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • RNA Editing / drug effects*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Apolipoproteins B
  • Insulin
  • RNA, Messenger
  • AICDA (activation-induced cytidine deaminase)
  • APOBEC-1 Deaminase
  • Apobec1 protein, rat
  • Cytidine Deaminase