Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast

EMBO J. 1998 Jul 15;17(14):4004-17. doi: 10.1093/emboj/17.14.4004.

Abstract

Phosphatidylinositol transfer proteins (PITPs) have been shown to play important roles in regulating a number of signal transduction pathways that couple to vesicle trafficking reactions, phosphoinositide-driven receptor-mediated signaling cascades, and development. While yeast and metazoan PITPs have been analyzed in some detail, plant PITPs remain entirely uncharacterized. We report the identification and characterization of two soybean proteins, Ssh1p and Ssh2p, whose structural genes were recovered on the basis of their abilities to rescue the viability of PITP-deficient Saccharomyces cerevisiae strains. We demonstrate that, while both Ssh1p and Ssh2p share approximately 25% primary sequence identity with yeast PITP, these proteins exhibit biochemical properties that diverge from those of the known PITPs. Ssh1p and Ssh2p represent high-affinity phosphoinositide binding proteins that are distinguished from each other both on the basis of their phospholipid binding specificities and by their substantially non-overlapping patterns of expression in the soybean plant. Finally, we show that Ssh1p is phosphorylated in response to various environmental stress conditions, including hyperosmotic stress. We suggest that Ssh1p may function as one component of a stress response pathway that serves to protect the adult plant from osmotic insult.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Membrane / metabolism
  • Cloning, Molecular
  • Cytosol / metabolism
  • Gene Expression Regulation, Plant / physiology*
  • Genes, Plant / genetics
  • Membrane Proteins*
  • Molecular Sequence Data
  • Osmolar Concentration
  • Phosphatidylinositols / metabolism*
  • Phospholipid Transfer Proteins
  • Phosphorylation
  • Plant Proteins / metabolism*
  • Protein Binding
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Sodium Chloride
  • Sorbitol
  • Soybeans / genetics*
  • Soybeans / metabolism

Substances

  • Carrier Proteins
  • Membrane Proteins
  • Phosphatidylinositols
  • Phospholipid Transfer Proteins
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Recombinant Fusion Proteins
  • SEC24 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sodium Chloride
  • Sorbitol

Associated data

  • GENBANK/AF024651
  • GENBANK/AF024652