Rapid fluorescence-based measurement of neutrophil migration in vitro

J Immunol Methods. 1998 Apr 1;213(1):41-52. doi: 10.1016/s0022-1759(98)00016-7.

Abstract

We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the fluorescence-based assay was more rapid, less labor intensive, and more sensitive. This new assay is a significant advance in the measurement of leukocyte migration in vitro.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Count
  • Chemotaxis, Leukocyte*
  • Evaluation Studies as Topic
  • Fluoresceins
  • Fluorescence
  • Fluorescent Dyes
  • Humans
  • Micropore Filters
  • Neutrophils / physiology*
  • Polycarboxylate Cement
  • Polyesters
  • Spectrometry, Fluorescence / methods*
  • Time Factors

Substances

  • Fluoresceins
  • Fluorescent Dyes
  • Polycarboxylate Cement
  • Polyesters
  • polycarbonate
  • fluorexon