A sandwich enzyme immunoassay for measuring AD7C-NTP as an Alzheimer's disease marker: AD7C test

J Clin Lab Anal. 1998;12(4):223-6. doi: 10.1002/(sici)1098-2825(1998)12:4<223::aid-jcla6>3.0.co;2-8.

Abstract

A simple, fast, reliable, and specific immunoassay has been developed to detect and measure AD7C-NTP, a biochemical marker for Alzheimer's disease, in cerebrospinal fluid (CSF). This assay, called the AD7C Test, is an enzyme-linked sandwich immunoassay (ELSIA) using 96 well microtiter plates. The plate surface is coated with a monoclonal antibody (N3I4) which has a high affinity and specificity for AD7C-NTP, capturing it effectively from CSF samples. The detection was achieved using a polyclonal antibody (ADRI). Both N3I4 and ADRI were generated using recombinantly produced AD7C-NTP. The assay is highly sensitive (30-50 pg), linear to 2.0 ng (r2 > 0.99), and reproducible (C.V. < 10%). The utility of the assay has been demonstrated using CSF specimens from early Alzheimer's disease patients and age matched controls (sensitivity of 89% and specificity of 89%).

Publication types

  • Clinical Trial

MeSH terms

  • Alzheimer Disease / cerebrospinal fluid*
  • Alzheimer Disease / diagnosis
  • Antibodies / immunology
  • Antibodies, Monoclonal / immunology
  • Biomarkers / cerebrospinal fluid
  • Humans
  • Immunoenzyme Techniques
  • Nerve Tissue Proteins / cerebrospinal fluid*
  • Nerve Tissue Proteins / immunology
  • Recombinant Proteins / cerebrospinal fluid
  • Recombinant Proteins / immunology
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • AD7c-NTP
  • Antibodies
  • Antibodies, Monoclonal
  • Biomarkers
  • Nerve Tissue Proteins
  • Recombinant Proteins