Regulation of granulocyte colony-stimulating factor gene expression by interleukin-17

Immunol Lett. 1998 May;62(1):51-8. doi: 10.1016/s0165-2478(98)00027-3.


Interleukin-17 (IL-17) has been previously reported to induce stromal cells to produce a number of hematopoietic and proinflammatory cytokines, including granulocyte colony-stimulating factor (G-CSF). Here, we have evaluated the mechanisms responsible for the augmentation of G-CSF gene expression by IL-17, using the murine 3T3 fibroblast cell line. Treatment of 3T3 cells, but not primary bone marrow-derived macrophages or murine monocyte/macrophage cell lines, resulted in increased steady-state G-CSF mRNA levels within 2-4 h and augmented G-CSF protein production. The combination of IL-17 and LPS enhanced G-CSF expression in an additive fashion. Stability studies revealed that IL-17 stabilized G-CSF mRNA levels, with a t1/2 of 4 h, compared to a t1/2 of less than 2 h in medium or LPS-treated cells. Induction of G-CSF expression in 3T3 cells by IL-17 did not appear to require tyrosine kinase activation or de novo protein synthesis. These studies indicate that post-transcriptional mechanisms play an important role in IL-17-induced G-CSF expression in fibroblasts and suggest that IL-17 may be useful for further delineating mechanisms of G-CSF gene regulation.

MeSH terms

  • 3T3 Cells
  • Animals
  • Cell Line
  • Cycloheximide / pharmacology
  • Cytokines / pharmacology*
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation / drug effects*
  • Genistein / pharmacology
  • Granulocyte Colony-Stimulating Factor / genetics*
  • Interleukin-17
  • Interleukins / pharmacology*
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Mitogens / pharmacology
  • Protein Synthesis Inhibitors / pharmacology


  • Cytokines
  • Enzyme Inhibitors
  • Interleukin-17
  • Interleukins
  • Lipopolysaccharides
  • Mitogens
  • Protein Synthesis Inhibitors
  • Granulocyte Colony-Stimulating Factor
  • Cycloheximide
  • Genistein