Engineering the luxCDABE Genes From Photorhabdus Luminescens to Provide a Bioluminescent Reporter for Constitutive and Promoter Probe Plasmids and mini-Tn5 Constructs

FEMS Microbiol Lett. 1998 Jun 15;163(2):193-202. doi: 10.1111/j.1574-6968.1998.tb13045.x.

Abstract

The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Conjugation, Genetic
  • DNA Transposable Elements*
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae / growth & development
  • Gene Expression Regulation, Bacterial*
  • Genes, Reporter / genetics*
  • Luminescent Measurements
  • Operon*
  • Photometry
  • Plasmids / genetics*
  • Promoter Regions, Genetic*
  • Restriction Mapping
  • Signal Transduction

Substances

  • DNA Transposable Elements