A Subset of TAF(II)s Are Integral Components of the SAGA Complex Required for Nucleosome Acetylation and Transcriptional Stimulation

Cell. 1998 Jul 10;94(1):45-53. doi: 10.1016/s0092-8674(00)81220-9.

Abstract

A number of transcriptional coactivator proteins have been identified as histone acetyltransferase (HAT) proteins, providing a direct molecular basis for the coupling of histone acetylation and transcriptional activation. The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex requires the coactivator protein Gcn5 for HAT activity. Identification of protein subunits by mass spectrometry and immunoblotting revealed that the TATA binding protein-associated factors (TAF(II)s) TAF(II)90, -68/61, -60, -25/23, and -20/17 are integral components of this complex. In addition, TAF(II)68 was required for both SAGA-dependent nucleosomal HAT activity and transcriptional activation from chromatin templates in vitro. These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / metabolism*
  • Histone Acetyltransferases
  • Histones / metabolism
  • Nucleosomes / metabolism*
  • Protein Kinases / metabolism
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • TATA-Binding Protein Associated Factors*
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Histones
  • NGG1 protein, S cerevisiae
  • Nucleosomes
  • SPT3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • TAF12 protein, S cerevisiae
  • TATA-Binding Protein Associated Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors
  • Acetyltransferases
  • GCN5 protein, S cerevisiae
  • Histone Acetyltransferases
  • Protein Kinases